注意:因业务调整,暂不接受个人委托测试望见谅。
品系全名:C57BL/6JSmoc-Tg(CAG-LSL-dCas9-SPH)Smoc
目录号:NM-TG-00025
品系状态:活体
Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, 3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, A-11034, Thermo Fisher).
基因名:dCas9
基因曾用名:Null
NCBI ID:Null
MGI ID:Null
Ensembl ID:Null
Pubmed:dCas9
暂无数据
1.具体的试验周期以工程师告知的为准。
2.文章中的图片或者标准以及具体的试验方案仅供参考,因为每个样品和项目都有所不同,所以最终以工程师告知的为准。
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4.加急试验周期一般是五个工作日左右,部分样品有所差异
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