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R26-CAG-LSL-RSR-tdTomato-2A-DTR

原创发布者:北检院    发布时间:2023-03-16     点击数:

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品系介绍

品系全名:C57BL/6JSmoc-Gt(ROSA)26Sorem1(CAG-LSL-RSR-tdTomato-2A-DTR)Smoc

目录号:NM-KI-190086

品系状态:活体

将CAG-LSL-RSR-tdTomato-2A-DTR插入到小鼠Rosa26基因中。

基本信息

基因名:Gt(ROSA)26Sor

基因曾用名:R26,ROSA26,AV258896,Gtrgeo26,Gtrosa26,Thumpd3as1

NCBI ID:14910

MGI ID:104735

Ensembl ID:ENSMUSG00000086429

Pubmed:Gt(ROSA)26Sor

相关数据

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Fig1. Immunostaining for tdTomato on sections of multiple tissues or organs collected from ACTB-Cre;R26-LR-tdT-DTR mice (upper panel) or CAG-Dre;R26-LR-tdT-DTR mice (lower panel). 

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Fig2. DTR-mediated cardiomyocyte ablation after DT administration. 

A, a schematic figure showing experimental design. B, whole-mount view of triple transgenic mice treated with PBS or DT. Yellow scale bar represents 2000 μm.  C, immunostaining for DTR and tdTomato on heart sections of triple transgenic mice. White scale bar represents100 μm.  D, immunostaining for cTnT and tdTomato on heart sections of triple transgenic mice. White scale bar represents 100 μm. E, immunostaining for Tunel and tdTomato on heart sections. White scale bar represents 100 μm.

image.png

Fig3. DTR-mediated specific ablation of Cyp2e1+ peri-central hepatocytes.

A, a cartoon figure showing the mouse’s mating strategy. B, whole-mount epifluorescence view of livers collected from mice treated with DT or PBS. Yellow scale bar represents 2000 μm.  C and D, immunostaining for tdTomato, HNF4a (E), or DTR (F) on liver sections treated with PBS. White scale bar represents 100 μm. C, central vein. E, immunostaining for CYP2E1, tdTomato, and E-cad on liver sections from mice treated with PBS or DT. White scale bar represents 100 μm. F, quantification of the percentage of tdTomato+ hepatocytes (DT group) in comparison of tdTomato+ hepatocytes (PBS group). G, immunostaining for Tunel and tdTomato on liver sections from mice treated with PBS or DT. White scale barrepresents 100 μm.

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Fig4. DTR-mediated specific ablation of Mfsd2a+ peri-portal hepatocytes.

A, a schematic figure showing the genetic recombination of the R26-LR-tdT-DTR allele by Mfsd2a-CreER and AAV8-TBG-Dre.  B, whole-mount fluorescent view of livers collected from mice treated with PBS or DT. Yellow scale bar represents 2000 μm.  C and D, immunostaining for tdTomato, HNF4a (E), or DTR (F) on liver sections of mice treated with PBS.White scale bar represents100 μm. E, immunostainingfor E-cad and tdTomato on liver sections from mice treated with PBS or DT. White scale bar represents 100 μm.  F, quantification of the percentage of tdTomato+ hepatocytes (DT group) in comparison with tdTomato+ hepatocytes (PBS group). n = 5.  G, immunostaining for Tunnel and tdTomato on liver sections of mice treated with PBS or DT. White scale bar represents 100 μm.

*以上数据源自:Wang H, He L, Li Y, et al. Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo. J Biol Chem. 2022;298(6):101965. doi:10.1016/j.jbc.2022.101965

相关品系

R26-CAG-LSL-SLIT3-R1479C品系名:C57BL/6JSmoc-Gt(ROSA)26Sorem1(CAG-LSL-SLIT3-R1479C-WPRE-pA)Smoc品系状态:活体 | 目录号:NM-KI-225092

R26-CAG-LSL-hMSLN-IRES-Luc品系名:C57BL/6JSmoc-Gt(ROSA)26Sorem1(CAG-LSL-hMSLN-IRES-Luciferase-WPRE-pA)Smoc品系状态:在研 | 目录号:NM-KI-225094

R26-SA-LNL-Pik3ca-H1047R品系名:C57BL/6JSmoc-Gt(ROSA)26Sorem1(SA-Loxp-PGK-Neo-pA-Loxp-Pik3ca-H1047R-pA)Smoc品系状态:在研 | 目录号:NM-KI-225095

R26-SA-LSL-hMycRTG3-ERT2-IRES-EGFP品系名:C57BL/6JSmoc-Gt(ROSA)26Sorem1(SA-loxp-stop-loxp-hMycRTG3-ERT2-IRES-EGFP-WPRE-pA)Smoc品系状态:精子冻存 | 目录号:NM-KI-225096

R26-CAG-DIO-Cre-2A-GFP品系名:C57BL/6JSmoc-Gt(ROSA)26Sortm1(CAG-DIO-iCre-P2A-GFP-wpre-polyA-Frt-Neo-Frt)Smoc品系状态:在研 | 目录号:NM-KI-230180

实验仪器

实验室仪器 实验室仪器 实验室仪器 实验室仪器

测试流程

<p><b>基因名:</b>Gt(ROSA)26Sor</p>流程

注意事项

1.具体的试验周期以工程师告知的为准。

2.文章中的图片或者标准以及具体的试验方案仅供参考,因为每个样品和项目都有所不同,所以最终以工程师告知的为准。

3.关于(样品量)的需求,最好是先咨询我们的工程师确定,避免不必要的样品损失。

4.加急试验周期一般是五个工作日左右,部分样品有所差异

5.如果对于(R26-CAG-LSL-RSR-tdTomato-2A-DTR)还有什么疑问,可以咨询我们的工程师为您一一解答。

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