蛋白表达检测

原创发布者：北检院发布时间：2024-01-21 点击数：

注意：因业务调整，暂不接受个人委托测试望见谅。

蛋白表达检测范围

细胞培养, 转染, 原核表达, 克隆, 免疫沉淀, 裂解, 融合蛋白, 纯化, 重组蛋白, Western blot, SDS-PAGE, 酵母表达, 化学合成, 功能研究, 稳定细胞株, 转录, 翻译, 具体呈现, 蛋白交互, 结构解析, 抗体制备

蛋白表达检测项目

PCR, Western blot, Immunohistochemistry, Immunofluorescence, Flow cytometry, ELISA, Mass spectrometry, Protein purification, Protein quantification, Protein crystallization, Protein-protein interaction assays, Protein structure determination, Protein folding, Protease activity assay, Protein degradation assay, Protein phosphorylation assay, Protein acetylation assay, Protein glycosylation assay, Protein methylation assay, Protein ubiquitination assay, Protein kinase assay, Protein phosphatase assay, Protein-protein crosslinking assay, Protein alignment, Protein sequence analysis, Protein stability assay, Protein function assay, Protein engineering, Protein localization, Protein trafficking, Protein degradation, Protein aggregation, Protein cleavage, Protein expression analysis, Protein labeling, Protein-protein interaction analysis, Protein-DNA interaction analysis, Protein-RNA interaction analysis, Protein-lipid interaction analysis, Protein-protein crosslinking analysis, Protein secondary structure analysis, Protein tertiary structure analysis, Protein quaternary structure analysis, Protein dynamics analysis, Protein folding analysis, Protein stability analysis, Protein-protein docking, Protein-ligand docking, Protein-protein interaction prediction, Protein structure prediction, Protein modeling, Protein network analysis, Protein evolution analysis, Protein phosphorylation site prediction, Protein subcellular localization prediction, Protein sequence motif analysis, Protein sequence alignment, Protein domain prediction, Protein functional annotation.

蛋白表达检测方法

Western blotting: Western blotting is a commonly used method to detect and analyze protein expression. It involves separating proteins using gel electrophoresis, transferring them to a membrane, and then probing the membrane with specific antibodies that bind to the target protein. This method allows for the detection and quantification of protein expression levels.

Immunohistochemistry (IHC): Immunohistochemistry is a technique used to visualize protein expression in tissue samples. It involves using specific antibodies that bind to the protein of interest, followed by the addition of a colored or fluorescent tag that can be visualized under a microscope. IHC is useful for determining the localization and distribution of proteins within tissues.

Immunofluorescence (IF): Immunofluorescence is a similar technique to IHC but uses fluorescent tags instead of colored tags. This allows for the visualization of protein expression in cells or tissues using fluorescence microscopy. Compared to IHC, IF provides higher sensitivity and allows for the simultaneous detection of multiple proteins using different fluorescent tags.

Enzyme-Linked Immunosorbent Assay (ELISA): ELISA is a quantitative method used to measure the concentration of proteins in a sample. It relies on the specific binding of antibodies to the target protein and the use of an enzyme-conjugated secondary antibody to generate a signal that can be measured. ELISA is commonly used in research, diagnostics, and drug discovery for protein quantification.

Protein gel electrophoresis: Protein gel electrophoresis is a technique used to separate proteins based on their size and charge. It involves running the protein sample through a gel matrix, typically made of polyacrylamide, under an electric field. The proteins move through the gel at different rates based on their size and charge, allowing for their separation. This method is often used as a preliminary step before other protein analysis techniques.

Mass spectrometry: Mass spectrometry is a powerful tool used to identify and quantify proteins in a sample based on their mass-to-charge ratio. It involves ionizing the proteins and measuring their mass-to-charge ratio using a mass spectrometer. This technique can provide information about the identity, sequence, and modifications of proteins present in a sample.

Reverse transcription-polymerase chain reaction (RT-PCR): RT-PCR is a method used to detect and quantify the expression of specific genes. It involves reverse transcribing RNA into complementary DNA (cDNA) using reverse transcriptase, followed by amplification of the target cDNA using PCR. By designing specific primers, RT-PCR can be used to measure the expression levels of specific genes of interest.

Flow cytometry: Flow cytometry is a technique used to analyze protein expression in individual cells within a population. It involves staining cells with fluorescently-labeled antibodies that bind to specific proteins of interest. The stained cells are then passed through a flow cytometer, which measures the fluorescence intensity of each individual cell. Flow cytometry allows for the assessment of protein expression at the single-cell level and can provide information about cell populations and their characteristics.

Proximity ligation assay (PLA): PLA is a method used to detect protein-protein interactions. It relies on the binding of two antibodies to the proteins of interest, followed by the ligation of DNA strands attached to the antibodies. The ligated DNA strands are then amplified using PCR and visualized using fluorescence microscopy. PLA can provide information about the spatial and temporal co-localization of proteins involved in specific interactions.

Immunoprecipitation (IP): Immunoprecipitation is a technique used to isolate and purify a specific protein or protein complex from a mixture. It involves the use of antibodies that specifically bind to the protein of interest. The antibody-protein complex is then precipitated using protein A or protein G beads. This method allows for the characterization and analysis of protein-protein interactions, post-translational modifications, and protein function.